Protocol for immunodetection of DNA-protein crosslinks and their PTMs

https://pmc.ncbi.nlm.nih.gov/articles/PMC12701772/pdf/nihms-2117765.pdf

Protocol for real-time imaging of trapping of cellular topoisomerases and PARPs at the single-molecule level

https://pmc.ncbi.nlm.nih.gov/articles/PMC12701772/pdf/nihms-2117765.pdf

Plasmids

Human TOP1, TOP1MT, TOP2A, TOP2B, TOP3A, TOP3B, PARP1 and SLFN11-HaloTag/SnapTag expression plasmids 

FLAG-tagged  human TOP1 T718A/N722H, TOP2A D48N, and TOP2B D64N self-trapping mutant expression plasmids

Cell lines

Topoisomerase-knockout human cancer cell lines and others